CD106 (VCAM-1), clone: STA, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1069-80

Product Code. 15297157

Description

Description: The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to Integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1). Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.Applications Reported: STA has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: The STA antibody has been tested by flow cytometric analysis of TNF alpha-activated HUVEC cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD106 (VCAM-1)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P19320
• Gene Alias: CD106, DKFZp779G2333, INCAM-100, MGC99561, CD106 antigen, vascular cell adhesion protein 1
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow CytometryþImmunohistochemistry (Frozen)
• Clone: STA
• Conjugate: Unconjugated
• Gene: VCAM1
• Format: Purified
• Gene Symbols: VCAM1
• Isotype: IgG1, kappa
• Quantity: 25μg
• Storage Requirements: Store at 2-8°C.
• Primary or Secondary: Primary
• Gene ID (Entrez): 7412

For Research Use Only.