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CD103 (Integrin alpha E), PerCP-eFluor™ 710, clone: Ber-ACT8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-1037-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15589166

  • £77.45 / 25 tests


EU Stock 2
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Description

Description

Description: The Ber-ACT8 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen (E-cadherin) binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium. Cross-blocking studies suggest that B-Ly7 and Ber-ACT8 see different epitopes.Applications Reported: This Ber-ACT8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This Ber-ACT8 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD103 (Integrin alpha E)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P38570
ITGAE, HUMINAE
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
Ber-ACT8
PerCP-eFluor 710
ITGAE
Conjugated
ITGAE
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3682
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