CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Biotin, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-1031-81

Product Code. 15239669

Description

Description: The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7 and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium. Applications Reported: This 2E7 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: The 2E7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs.

Specifications

• Antigen: CD103 (Integrin alpha E)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q60677
• Gene Symbols: Itgae
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16407
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen)
• Clone: 2E7
• Conjugate: Biotin
• Gene: Itgae
• Gene Alias: ITGAE, HUMINAE
• Host Species: Armenian Hamster
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

For Research Use Only.