CD101 Monoclonal Antibody (BB27), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1019-80

Product Code. 15237137

Description

Description: The monoclonal antibody BB27 reacts to human CD101 also known as V7 antigen, a member of the Ig superfamily. CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population. Applications Reported: This BB27 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining(frozen tissue). Applications Tested: This BB27 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test.

CD101 also known as V7 antigen, a member of the Ig superfamily. CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population.

Specifications

• Antigen: CD101
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q93033
• Gene Symbols: CD101
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9398
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: BB27
• Conjugate: Unconjugated
• Gene: CD101
• Gene Alias: Cd101; CD101 antigen; CD101 molecule; cell surface glycoprotein V7; EWI immunoglobulin subfamily member EWI-101; EWI101; EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; Gm1016; Gm734; IGSF2; Immunoglobulin superfamily member 2; immunoglobulin superfamily, member 2; leukocyte surface protein; RP11-27K13.2; V7
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

For Research Use Only.