CD100 (SEMA4D), clone: eBio133-1C6 (133-1C6), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1009-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D. CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. Furthermore CD100 is a costimulatory molecule in adaptive immune responses by switching off negative signalling from CD72. In addition to CD72 another ligand is Plexin-B1. It has been shown that higher expression levels of CD100 are correlated with poor survival in soft tissue sarcoma patients. Expression is found in both hematopoietic and non-hematopoietic cells.CD100 is expressed on resting T cells and NK cells and B cell. CD100 can also exist as a soluble form.Applications Reported: This eBio133-1C6 (133-1C6) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and ELISA.Applications Tested: This eBio133-1C6 (133-1C6) antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|A8, BB18, GR3, Leukocyte activation antigen CD100, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D|
|Store at 2-8°C.|
For Research Use Only.