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CD100 (SEMA4D), clone: eBio133-1C6 (133-1C6), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1009-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15227137

  • £175.46 / 100µg


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Description

Description

Description: The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D. CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. Furthermore CD100 is a costimulatory molecule in adaptive immune responses by switching off negative signalling from CD72. In addition to CD72 another ligand is Plexin-B1. It has been shown that higher expression levels of CD100 are correlated with poor survival in soft tissue sarcoma patients. Expression is found in both hematopoietic and non-hematopoietic cells.CD100 is expressed on resting T cells and NK cells and B cell. CD100 can also exist as a soluble form.Applications Reported: This eBio133-1C6 (133-1C6) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and ELISA.Applications Tested: This eBio133-1C6 (133-1C6) antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD100 (SEMA4D)
eBio133-1C6 (133-1C6)
Unconjugated
Purified
Murine
100μg
Store at 2-8°C.
Monoclonal
Immunoprecipitation
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Semaphorin 4D
IgM
RUO
Primary
Human
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