missing translation for 'onlineSavingsMsg'
Learn More

CD10, PerCP-eFluor™ 710, clone: SN5c, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0108-42

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15529076

  • £231.47 / 100 tests


Estimated Shipment date
from Supplier 29-05-2018
Add to basket

Description

Description

Description: The eBioSN5c monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALLs). Subsequently, CD10 was shown to be the same molecule as neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn^2+-dependent metallopeptidase acting on substrates such as endothelin, glucagon, gastrin, neurotensin and bradykinin. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and its expression has been reported on mature germinal center B cells. CD10 expression is also found on normal donor granulocytes and bone marrow stromal cells. Most likely, CB-CALLA and eBioSN5c see different epitopes due to their ability to co-stain.Applications Reported: This eBioSN5c (SN5c L4-1A1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This SN5c antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD10
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P08473
CALLA, CD10, NEP, SFE, atriopeptidase, common acute lymphocytic leukemia antigen, enkephalinase, membrane metallo-endopeptidase neutral endopeptidase, enkephalinase, membrane metallo-endopeptidase neutral endopeptidase, enkephalinase, CALLA, CD10, membrane metallo-endopeptidase
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
SN5c
PerCP-eFluor 710
MME
Conjugated
MME
IgG1
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
4311
Videos

For Research Use Only.