CD10 Monoclonal Antibody (eBioCB-CALLA (CB-CALLA)), PE-Cyanine5, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0106-41

Product Code. 15587706

Description

Description: The eBioCB-CALLA monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), which is a 100 kDa, type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALL). Subsequently, CD10 was shown to be the same molecule as the neutral endopeptidase (NEP), or KII-NA. CD10 is a Zn2+-dependent metallo-peptidase with endothelin, glucagon, gastrin, neurotensin and bradykinin included among its substrates. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils. In B cells, CD10 regulates stromal cell-dependent B lymphopoiesis and expression has also been reported on mature B cells in germinal centres. In addition to the hematopoietic compartment, other major sites of CD10 expression are the brush border of enterocytes and renal tubules and glomeruli. There is partial blocking of the eBioCB-CALLA and MEM-78 monoclonal antibodies indicating that they recognize similar epitopes. Applications Reported: This eBioCB-CALLA (CB-CALLA) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioCB-CALLA (CB-CALLA) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. CD10 (Common Acute Lymphocytic Leukemia Antigen ,CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt′s, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. CD10 is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. Further, CD10 is a 100 kDa type II transmembrane glycoprotein that exists in a single copy of greater than 45 kb. The 5′ untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts and the coding region is not affected by alternative splicing. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie tooth Disease.

Specifications

• Antigen: CD10
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P08473
• Gene Symbols: MME
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4311
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBioCB-CALLA (CB-CALLA)
• Conjugate: PE-Cyanine5
• Gene: MME
• Gene Alias: CALLA, CD10, NEP, SFE, atriopeptidase, common acute lymphocytic leukemia antigen, enkephalinase, membrane metallo-endopeptidase neutral endopeptidase, enkephalinase, membrane metallo-endopeptidase neutral endopeptidase, enkephalinase, CALLA, CD10, membrane metallo-endopeptidase
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.