missing translation for 'onlineSavingsMsg'
Learn More

CD10 Mouse anti-Human, PE-Cyanine5, Clone: eBioCB-CALLA (CB-CALLA), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0106-42

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15597706

  • £171.00 / 100 tests

Estimated Shipment
04-11-2020

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. CD10 (Common Acute Lymphocytic Leukemia Antigen ,CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt′s, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. CD10 is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. Further, CD10 is a 100 kDa type II transmembrane glycoprotein that exists in a single copy of greater than 45 kb. The 5′ untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts and the coding region is not affected by alternative splicing. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie tooth Disease.
Specifications

Specifications

CD10
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P08473
CALLA, CD10, NEP, SFE, atriopeptidase, common acute lymphocytic leukemia antigen, enkephalinase, membrane metallo-endopeptidase neutral endopeptidase, enkephalinase, membrane metallo-endopeptidase neutral endopeptidase, enkephalinase, CALLA, CD10, membrane metallo-endopeptidase
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
eBioCB-CALLA (CB-CALLA)
PE-Cyanine5
MME
Liquid
MME
IgG2b, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
4311
Videos
Documents

For Research Use Only.