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CCL3 (MIP-1 alpha), FITC, clone: CR3M, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-9706-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15566626

  • £175.12 / 100 tests


Estimated Shipment date
from Supplier 25-05-2018
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Description

Description

Description: This CR3M monoclonal antibody reacts with human CCL3. CCL3, also known as MIP-1 alpha (Macrophage Inflammatory Protein 1 alpha), is a member of the CC- subfamily of chemokines and is most closely related to CCL4, or MIP-1 beta. These proteins play critical roles in the recruitment of leukocytes to the site of inflammation. While both CCL3 and CCL4 will attract monocytes, macrophages, and dendritic cells, CCL3 preferentially attracts CD8+ T cells, while CD4+ T cells are more responsive to CCL4. In addition to its chemotactic functions, CCL3 induces inflammatory cytokine secretion, mast cell degranulation, and NK cell activation. It has also been reported to inhibit hematopoetic stem cell proliferation and may be responsible for the maintenance of these cells in a quiescent state.CCL3 signaling is mediated by the G protein-coupled receptors CCR1, CCR4, and CCR5, which are shared with CCL4 and CCL5 (RANTES). CCR5 is the primary co-receptor for HIV entry, which the virus binds through the gp120 envelope protein. All CCR5 ligands demonstrate potent inhibition of virus entry into the cell, both through steric hindrance of gp120-CCR5 interaction, and ligand-mediated receptor internalization.This CR3M clone is specific to CCL3, with no detectable cross-reactivity to Human CCL4 (MIP-1 beta).Applications Reported: This CR3M antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This CR3M antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CCL3 (MIP-1 alpha)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P10147
RP23-320E6.7, AI323804, G0S19-1, LD78alpha, MIP-1alpha, MIP1-(a), MIP1-alpha, Mip1a, Scya3
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
CR3M
FITC
CCL3
Conjugated
CCL3
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
6348
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