missing translation for 'onlineSavingsMsg'
Learn More

CCL2 (MCP-1), clone: 5D3-F7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7099-85

Code : Z2

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15208017

  • £149.74 / 500µg


Call for latest availability
+44 (0)1509 555 500
Add to basket

Description

Description

Description: The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.Applications Reported: The 5D3-F7 antibody has been reported to be useful for detection of MCP-1 in a sandwich ELISA, neutralization of MCP-1 bioactivity, intracellular staining for flow cytometric analysis, IP, and immunohistochemical staining of frozen tissue sections.Applications Tested: The affinity purified 5D3-F7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the biotinylated 2H5 (13-7096) antibody for detection and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.The Functional Grade purified 5D3-F7 antibody has been tested for neutralization of MCP-1 bioactivity.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CCL2 (MCP-1)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P13500
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
ELISAþFlow CytometryþImmunohistochemistryþImmunoprecipitation
5D3-F7
Unconjugated
CCL2
Purified
CCL2
IgG1, kappa
500μg
Store at 2-8°C.
Primary
6347
Videos

For Research Use Only.