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CCL2 (MCP-1), Biotin, clone: 2H5, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-7096-85

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15340030

  • £176.05 / 500µg


Estimated Shipment date
from Supplier 28-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5, cat. 16-7096, in functional assays.).Applications Tested: Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (14-7091) antibody for capture and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (14-7099) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.The Functional Grade purified 2H5 antibody has been tested for neutralization of MCP-1 bioactivity.The fluorochrome-conjugated 2H5 antibody has been tested for intracellular staining and flow cytometric analysis of mouse, rat, and human cells and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CCL2 (MCP-1)
2H5
Biotin
Conjugated
Armenian Hamster
500μg
Store at 2-8°C. Do not freeze.
Monoclonal
ELISA Detection
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
MCP1, MCAF, CCL-2
IgG
RUO
Primary
Rat
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