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CCL2 (MCP-1), Biotin, clone: 2H5, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-7096-81

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15330030

  • £68.95 / 50µg


Estimated Shipment date
from Supplier 30-07-2018
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Description

Description

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5, cat. 16-7096, in functional assays.).Applications Tested: Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (14-7091) antibody for capture and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (14-7099) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.The Functional Grade purified 2H5 antibody has been tested for neutralization of MCP-1 bioactivity.The fluorochrome-conjugated 2H5 antibody has been tested for intracellular staining and flow cytometric analysis of mouse, rat, and human cells and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CCL2 (MCP-1)
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P13500, P14844, P10148
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurineþRat
ELISA
2H5
Biotin
CCL2
Conjugated
CCL2
IgG
50μg
Store at 2-8°C. Do not freeze.
Primary
6347
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