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Caspase 8, clone: 1H10, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9934-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15208257

  • £143.12 / 100µg


Estimated Shipment
28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The monoclonal antibody 1H10 recognizes human Caspase-8 also known as FLICE and MACH, a member of the cysteine-requiring aspartate protease family. Caspase-8 is an upstream caspase responsible for Fas and TNF-alpha mediated apoptosis. After activation of the Fas or TNF receptors by their ligands, the adaptor molecule FADD binds to the receptor and recruits caspase-8 to the membrane. The formation of this complex induces the cleavage and activation of caspase-8. The active form of caspase-8, made of a heterotetramer of p18 and p10, is released in the cytoplasm and activates downstream apoptotic proteases (caspase-3, -4, -6, -7, -9 and -10). Several (>;8) isoforms have been identified: 3 different pro-forms called Caspase-8a (55kDA), Caspase-8b (53kDa), and Caspase-8L which is procaspase-8 with an additional N-terminal extension of 59 aa. The 1H10 antibody recognizes the p55 and p53 proforms in addition to the p43 and p18 cleaved forms. Under reducing conditions, an additional band (possibly non-specific) around 35kDa may be present. Under nonreducing conditions, this band is absent.Applications Reported: This 1H10 antibody has been reported for use in immunoblotting (WB).Applications Tested: This 1H10 antibody has been tested by immunoblot analysis of cell lines. This can be used at 1-5 μg/mL It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Caspase 8
1H10
Unconjugated
Purified
Rat
100μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Caspase8
IgG2a
RUO
Primary
Human
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