Caspase 8 Rat anti-Human, Clone: 1H10, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-9934-82

100UG:ANTI-HUMAN CASPASE 8 PURIFIED 100 UG

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15208257

Quantity Price
1 £ 169.0 / 100µg
Estimated Shipment date
from Supplier 12-12-2016
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Description and Specification

Specification

Antigen Caspase 8
Applications Western Blotting
Clone 1H10
Concentration 0.5mg/mL
Conjugate Unlabeled
Format Purified
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Caspase8
Host Species Rat
Isotype IgG2a
Quantity 100μg
Regulatory Status RUO
Species Reactivity Human
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The monoclonal antibody 1H10 recognizes human Caspase-8 also known as FLICE and MACH, a member of the cysteine-requiring aspartate protease family. Caspase-8 is an upstream caspase responsible for Fas and TNF-α mediated apoptosis. After activation of the Fas or TNF receptors by their ligands, the adaptor molecule FADD binds to the receptor and recruits caspase-8 to the membrane. The formation of this complex induces the cleavage and activation of caspase-8. The active form of caspase-8, made of a heterotetramer of p18 and p10, is released in the cytoplasm and activates downstream apoptotic proteases (caspase-3, -4, -6, -7, -9 and -10). Several (> 8) isoforms have been identified: 3 different pro-forms called Caspase-8a (55kDA), Caspase-8b (53kDa), and Caspase-8L which is procaspase-8 with an additional N-terminal extension of 59 aa.
The 1H10 antibody recognizes the p55 and p53 proforms in addition to the p43 and p18 cleaved forms. Under reducing conditions, an additional band (possibly non-specific) around 35kDa may be present. Under nonreducing conditions, this band is absent.