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Caspase 1 Monoclonal Antibody (5B10), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£120.00 - £296.00

Specifications

Antigen Caspase 1
Clone 5B10
Concentration 0.5 mg/mL
Applications Western Blot
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15268187
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Affymetrix eBioscience
14-9832-80
25 μg
£120.00
25µg
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Estimated Shipment: 27-03-2024
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15278187
View Documents Promotion Details
Affymetrix eBioscience
14-9832-82
100 μg
£296.00
100µg
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Estimated Shipment: 25-04-2024
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Description

Description

Description: The monoclonal antibody 5B10 recognizes mouse Caspase-1, a member of the cysteine-requiring aspartate protease family, which plays a critical role in cell suicide known as apoptosis. In particular, it converts pro-IL-1beta to mature IL-1beta; hence it is also called ICE (interleukin-1beta converting enzyme). Upon activation Caspase-1 exists as a tetramer of two p20 and two p10 subunits. There are several isoforms of Caspase-1. The 5B10 antibody recognizes both the pro-form of about 42kDa and the active p20 form. Applications Reported: This 5B10 antibody has been reported for use in immunoblotting (WB). Applications Tested: This 5B10 antibody has been tested by western blot analysis of lysates prepared from the J774 cell line. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.
Specifications

Specifications

Caspase 1
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
CASP1; CASP-1; CASP1 nirs variant 1; caspase 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase1; caspase-1; Caspase-1 subunit p10; Caspase-1 subunit p20; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL1BC; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
CASP1
Primary
4° C
CASP1
5B10
Western Blot
Unconjugated
Rat
Mouse
P29452
12362
IgG2a
Affinity chromatography
Antibody
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