Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514

Membrane-permeable dye used to monitor changes in intracellular free calcium concentrations in the cell. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ≈520nm.

Brand:  Invitrogen™ 65-0859-70

Product Code. 15530607

Description

• Calcium binding affinity: Kd = 232 nM
• Molecular Weight: ≈1100 d
• Peak Excitation: 490nm
• Peak Emission: 514nm
• Reconstitute in high-quality, freshly opened DMSO
• Recommended concentration: 2 to 5 mM
• Once reconstituted, protect from light and store dessicated at −20°C; avoid freeze-thawing

Specifications

• Detection Method: Fluorescent
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Emission: 490⁄514
• Quantity: 10 x 50μg

• For Use With (Application): Calcium Assay
• Product Type: Dye
• Product Line: eBioscience™

For Research Use Only. Not for use in diagnostic procedures.