Invitrogen™ eBioscience™ Calcein AM Viability Dye (UltraPure Grade)

A membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye.

Brand:  Invitrogen™ 65-0853-39

Product Code. 15550597

Description

• Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye
• Apoptotic and dead cells with compromised cell membranes do not retain Calcein
• Calcein is optimally excited at 495 nm and has a peak emission of 515 nm
• For flow cytometric analysis, it can be excited off the blue laser line (488 nm) and detected using filters for FITC/Alexa Fluor™ 488 (530/30)
• For multicolor flow cytometry, concentrations in the range of 5 to 20 nM are recommended to ensure that compensation can be performed successfully
• Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
• If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854)
• Calcein AM should be reconstituted in high-quality, freshly opened DMSO
• Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time (avoid freeze-thawing)
• Purity: >95% (determined by HPLC)

Specifications

• Color: Green
• For Use With (Application): Viability Assay
• Product Type: Dye
• Product Line: eBioscience™

• Detection Method: Fluorescent
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Emission: 495/515
• Quantity: 1 mg

For Research Use Only. Not for use in diagnostic procedures.