eBioscience Calcein AM Viability Dye, UltraPure Grade

Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein.

Overview
Brand: eBioscience

Manufacturer Part Number: 65-0853-78

X20 Calcein AM Viability Dye (UltraPure Grade) 20x 50 ug

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only

Product Code. 15560597

Quantity Price
1 £ 195.6 / Pack of 20
Estimated Shipment date
from Supplier 20-01-2017
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Description and Specification

Specification

Quantity 20 x 50μg
Format Lyophilized off-white solid
For Use With (Application) Flow Cytometric Analysis, Microscopy
Storage Requirements −20°C. Protect from light and moisture.

  • Molecular weight: 994.86g/mol
  • Peak excitation: 495nm
  • Peak emission: 515nm
  • Reconstitute in high-quality, freshly opened DMSO
  • Once reconstituted, store at −20°C with dessicant and use within a short period of time. Avoid freeze-thawing
  • For fluorescent microscopy, detect using the appropriate filter sets
  • For flow cytometric analysis, excite with the blue laser line (488nm) and detect using filters for FITC/Alexa Fluor™ 488 (530/30)
  • Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
  • If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854)