c-Rel, PE, clone: 1RELAH5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-6111-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells.Applications Reported: This 1RELAH5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 1RELAH5 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3 Fixation/Permeabilization Buffers (cat. 00-5521) and protocol. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Flow CytometryþFlow Cytometry|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.