c-Rel, eFluor 660, clone: 1RELAH5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 50-6111-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells.Applications Reported: This 1RELAH5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 1RELAH5 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3 Fixation/Permeabilization Buffers (cat. 00-5521) and protocol. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.