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c-MAF, PerCP-eFluor™ 710, clone: sym0F1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9855-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15529446

  • £97.03 / 25 tests


Estimated Shipment date
from Supplier 04-05-2018
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Description

Description

Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This sym0F1 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 μL (0.0075 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

c-Maf
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O75444, P54843
2810401A20Rik, A230108G15Rik, AW047063, c-maf
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
sym0F1
PerCP-eFluor 710
MAF
Conjugated
MAF
IgG2b, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
4094
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