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MAF, PE, clone: sym0F1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9855-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15587246

  • £205.21 / 100 tests


EU Stock 2
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Description

Description

Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.Sym0F1 does not cross-react with MafA or MafB.Applications Reported: This sym0F1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This sym0F1 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

c-Maf
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P54843, O75444
2810401A20Rik, A230108G15Rik, AW047063, c-maf
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
HumanĂ¾Murine
Flow Cytometry
sym0F1
PE
MAF
Conjugated
Maf
IgG2b, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
4094
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