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c-MAF, eFluor 660, clone: sym0F1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9855-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15560147

  • £87.34 / 25µg


EU Stock 2
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Disclaimers:

For Research Use Only.


Description

Description

Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.Applications Reported: This sym0F1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This sym0F1 antibody has been tested by intracellular staining followed by flow cytometric analysis of TCR-activated mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

c-Maf
sym0F1
eFluor 660
Conjugated
Murine
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Maf
IgG2b
RUO
Primary
Murine
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