ErbB3 (HER-3), PerCP-eFluor™ 710, clone: SGP1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-6555-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: This SGP1 monoclonal antibody reacts with human c-ErbB3, which is also known as HER3. This epidermal growth factor (EGF) receptor family member is expressed on normal human tissues, particularly on neurons and cells of the gastrointestinal tract. c-ErbB3 is overexpressed in a variety of cancers, including breast, ovary, and gastrointestinal carcinomas. Upon binding its ligand heregulin beta-1, c-ErbB3 is activated by heterodimerization with ErbB2 or EGFR1. Dependent on PI3K signaling, c-ErbB3 mediates tumor cell invasion, intravasation, and metastasis. Expression of this cell surface receptor has been used as a prognostic marker for breast cancer.The SGP1 antibody has been reported to stimulate anchorage-independent growth of breast cancer cell lines.Applications Reported: This SGP1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SGP1 antibody has been pre-titrated and tested by flow cytometric analysis of the MCF7 cell line. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|