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BrdU Monoclonal Antibody (BU20A), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£262.00

Specifications

Antigen BrdU
Clone BU20A
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 1
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15509266
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Affymetrix eBioscience
46-5071-42
100 Tests
£262.00
100 tests
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Estimated Shipment: 07-05-2024
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Description

Description

Description: This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment. For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (cat. 00-5525) and protocol. Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This BU20A antibody has been pre-titrated and tested by flow cytometric analysis of BrdU-pulsed mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol or the BrdU Staining Buffer Set (cat. 00-5525) and protocol. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

BrdU Monoclonal antibody specifically detects BrdU in Chemical samples. It is validated for Flow Cytometry.
Specifications

Specifications

BrdU
5 μL/Test
Monoclonal
Liquid
RUO
IgG1 κ
Affinity chromatography
Antibody
BU20A
Flow Cytometry
PerCP-eFluor 710
Mouse
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Primary
4° C, store in dark, DO NOT FREEZE!
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