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BrdU, eFluor 450, clone: BU20A, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-5071-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15549756

  • £69.96 / 25 tests


Estimated Shipment date
from Supplier 04-06-2018
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Description

Description

Description: This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment. For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (cat. 00-5525) and protocol.Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This BU20A antibody has been pre-titrated and tested This BU20A antibody has been pre-titrated and tested by flow cytometric analysis of BrdU-pulsed mouse splenocytes using the BrdU Staining Buffer Set (cat. 00-5525) and protocol. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

BrdU
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
5-Bromo-2-deoxyuridine, Bromodeoxyuridine
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Rat
Flow Cytometry
BU20A
eFluor 450
Conjugated
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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