BrdU Monoclonal Antibody (BU20A), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-5071-42

Product Code. 15559756

Description

Description: This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment. For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (cat. 00-5525) and protocol. Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This BU20A antibody has been pre-titrated and tested This BU20A antibody has been pre-titrated and tested by flow cytometric analysis of BrdU-pulsed mouse splenocytes using the BrdU Staining Buffer Set (cat. 00-5525) and protocol. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

BrdU Monoclonal antibody specifically detects BrdU in Chemical samples. It is validated for Flow Cytometry.

Specifications

• Antigen: BrdU
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: BU20A
• Conjugate: eFluor 450
• Gene Alias: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid
• Target Molecule: Chemical

For Research Use Only.