Brachyury, clone: X1AO2, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9770-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody X1AO2 recognizes human Brachyury, a 47kDa protein encoded by the T gene and functioning as a nuclear transcription factor. This transcription factor binds to specific DNA regions near the palindromic sequence TCACACCT (T-site) through a region in its N-terminus, called the T-box. Brachyury functions during embryogenesis to regulate midline development by establishing the anterior/posterior axis through the regulation of genes involved in mesoderm formation and differentiation. Brachyury is the primary mesoderm marker used to distinguish differentiation of embryonic stem cells into a mesenchymal stem cell lineage. Variations in the T gene in humans are associated with neural tube defects and chordomas. Expression of brachyury is also increased in advanced stage lung tumors and tumor cell lines.Applications Reported: This X1AO2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry.Applications Tested: This X1AO2 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 μg/mL. This X1AO2 antibody has also been tested by immunocytochemistry of fixed and permeabilized human cells and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|RP11-459F1.1, TFT, T brachyury homolog, brachyury protein, protein T|
|Store at 2-8°C.|
For Research Use Only.