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CD249 (BP-1), PerCP-eFluor™ 710, clone: 6C3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5891-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15529306

  • £87.86 / 25µg


EU Stock 3
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Description

Description

Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.Applications Reported: This 6C3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

BP-1
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P16406
ENPEP, BP1, APA, gp160, AP-A, EAP, aminopeptidase A, differentiation antigen gp160, glutamyl aminopeptidase
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
6C3
PerCP-eFluor 710
Enpep
Conjugated
Enpep
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
13809
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