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CD249 (BP-1), clone: 6C3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5891-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15267567

  • £37.33 / 50Āµg


Estimated Shipment
06-07-2018
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Description

Description

Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.Applications Reported: The 6C3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.Applications Tested: The 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

BP-1
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P16406
ENPEP, BP1, APA, gp160, AP-A, EAP, aminopeptidase A, differentiation antigen gp160, glutamyl aminopeptidase
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryĆ¾ImmunohistochemistryĆ¾Immunoprecipitation
6C3
Unconjugated
Enpep
Purified
Enpep
IgG2a, kappa
50μg
Store at 2-8°C.
Primary
13809
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