BMI-1 Mouse anti-Human, Clone: AF27, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-9702-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15517616

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Description and Specification

Specification

Antigen BMI-1
Applications Immunocytochemistry
Applications Immunohistochemistry (Frozen)
Applications Immunoprecipitation
Applications Microscopy
Clone AF27
Concentration 0.5mg/mL
Conjugate Unlabeled
Format Purified
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Murine
Isotype IgG2a
Quantity 25μg
Regulatory Status RUO
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Target Species Human

The monoclonal antibody AF27 recognizes human BMI-1 (B cell-specific Moloney murine leukemia virus integration site 1), a member of the polycomb group (PcG) proteins responsible for repression of gene expression through recognition and modification of histone methylation and chromatin structure. BMI-1 contains a ring finger domain, a helix-turn-helix domain necessary for inducing telomerase activity and is a member of the polycomb repressive complex 1 (PRC1). BMI-1 localizes to the nucleus and plays an important role in self-renewal of normal as well as hematopoietic, embryonic, neural, and other stem cell populations through regulation of genes involved in proliferation such as Sox2 and KLF4. Decreased BMI-1 expression is associated with differentiation, while overexpression is frequently found in variety of cancers. BMI-1 has been shown to be essential for epithelial to mesenchymal transition (EMT) in head and neck and breast cancers and elevated BMI-1 expression correlates with poor prognosis in patients with neuroblastoma, glioma, non-Hodgkin B-cell lymphoma, oropharyngeal squamous cell cancer, non-small cell lung cancer, and primary and metastatic melanoma.