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Blimp-1, PE, clone: 5E7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9850-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15597236

  • £229.79 / 100µg


EU Stock 1
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Description

Description

Description: This 5E7 monoclonal antibody recognizes mouse B lymphocyte-induced maturation protein-1 (Blimp-1), which is also known as Prdm1. Blimp-1 is a transcriptional repressor with five zinc finger domains. Blimp-1 is expressed by terminally differentiating B and T cells. Blimp-1 is also expressed by some dendritic cells, and some NK cells. This transcriptional repressor is required for B cell differentiation into plasma cells and serves as a critical antagonist of CD4+ follicular helper T cell differentiation. While Blimp-1 is not required for priming and development of T and B cell effector function, it is critical for effector cell terminal differentiation. Blimp-1 promotes effector molecule secretion while suppressing proliferation. Blimp-1 also acts as a transcriptional antagonist of Bcl-6 and inhibits genes that are related to memory cell differentiation.Applications Reported: The 5E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: The 5E7 antibody has been tested by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Blimp1
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q60636
BLIMP 1, B Lymphocyte Induced Maturation Protein 1, BLIMP1, Positive Regulatory Domain I Binding Factor 1, PR Domain Containing 1, PRDI BF1, PRDM1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
5.00E+07
PE
Prdm1
Conjugated
Prdm1
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12142
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