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beta Catenin, Alexa Fluor 488, clone: 15B8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-2567-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15560207

  • £87.25 / 25 tests


Estimated Shipment date
from Supplier 25-05-2018
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Description

Description

Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.Applications Reported: This 15B8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 15B8 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cell line using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523). This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

beta-Catenin
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P35222, Q02248
Cadherin associated protein, Catenin beta 1, CATNB, CTNNB
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
15B8
Alexa Fluor 488
CTNNB1
Conjugated
CTNNB1
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
1499
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