beta Catenin, Alexa Fluor 488, clone: 15B8, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 53-2567-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.Applications Reported: This 15B8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 15B8 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cell line using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523). This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Cadherin associated protein, Catenin beta 1, CATNB, CTNNB|
|Alexa Fluor 488|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.