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BCL6, APC, clone: BCL-DWN, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-5453-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15598116

  • £93.52 / 25µg


Estimated Shipment date
from Supplier 26-06-2018
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Description

Description

Description: This BCL-DWN monoclonal antibody reacts with mouse Bcl-6, which is also known as B-cell lymphoma 6. A member of the POZ family of transcriptional repressors, Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79-kDa protein is essential for germinal center B cell proliferation and survival. Moreover, during B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1.Applications Reported: This BCL-DWN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This BCL-DWN antibody has been tested by intracellular staining followed by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.03 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Bcl-6
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P41183
B-cell lymphoma 6 protein, BCL-6, Zinc finger protein 51, LAZ-3 protein, BCL-5, Zinc finger and BTB domain-containing protein 27, ZNF51, ZBTB27, LAZ3, BCL5, BCL6
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
BCL-DWN
APC
Bcl6
Conjugated
Bcl6
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12053
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