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Bcl-2, eFluor 660, clone: 10C4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-6992-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15500017

  • £156.06 / 100 tests


Estimated Shipment date
from Supplier 25-05-2018
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Description

Description

Description: The 10C4 monoclonal antibody reacts with mouse and rat Bcl-2, also known as B cell lymphoma 2. Bcl-2 is the founding member of the Bcl-2 family of evolutionarily-conserved proteins that regulate cell death/survival. Members of the Bcl-2 family include the pro-apoptotic proteins BAD, Bax, and Bim, and the anti-apoptotic proteins Bcl-2, Bcl-xL, and Mcl-1. Bcl-2 inhibits apoptosis by preventing the release of cytochrome c from the mitochondria. Mutation of Bcl-2 or overexpression of Bcl-2 due to chromosomal translocation has been identified as a contributing factor in numerous cancers. In developing thymocytes, Bcl-2 is expressed at high levels in CD4-CD8- double negative and in CD4+ and CD8+ single positive thymocytes; Bcl-2 expression is greatly diminished in CD4+CD8+ double positive thymocytes.Applications Reported: This 10C4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 10C4 antibody has been pre-titrated and tested by intracellular flow cytometric analysis of mouse splenocytes and thymocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Bcl-2
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P49950, P10417
AW986256, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
Flow Cytometry
10C4
eFluor 660
Bcl2
Conjugated
Bcl2
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12043
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