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Bcl-2, eFluor 450, clone: 10C4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-6992-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15529796

  • £168.34 / 100 tests


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 10C4 monoclonal antibody reacts with mouse and rat Bcl-2, also known as B cell lymphoma 2. Bcl-2 is the founding member of the Bcl-2 family of evolutionarily-conserved proteins that regulate cell death/survival. Members of the Bcl-2 family include the pro-apoptotic proteins BAD, Bax, and Bim, and the anti-apoptotic proteins Bcl-2, Bcl-xL, and Mcl-1. Bcl-2 inhibits apoptosis by preventing the release of cytochrome c from the mitochondria. Mutation of Bcl-2 or overexpression of Bcl-2 due to chromosomal translocation has been identified as a contributing factor in numerous cancers. In developing thymocytes, Bcl-2 is expressed at high levels in CD4-CD8- double negative and in CD4+ and CD8+ single positive thymocytes; Bcl-2 expression is greatly diminished in CD4+CD8+ double positive thymocytes.Applications Reported: This 10C4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 10C4 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at 5 μL (1.0 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Bcl-2
10C4
eFluor 450
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (1.0μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
B cell lymphoma 2
IgG1
RUO
Primary
Rat
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