BATF, PE, clone: MBM7C7, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-9860-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The MBM7C7 monoclonal antibody reacts with human and mouse BATF (B cell activating transcription factor). BATF is a 14kDa member of the AP-1 family of transcription factors and is involved in the repression of other AP-1 family members. BATF is upregulated in activated T cells when compared to naive T and B cells and has been shown to be critical for Th17, but not Th1 or Th2 differentiation. In Th17, BATF interacts with the promoters of IL17, IL17F, IL21 and IL22. BATF has also been shown to be involved in CD8 T cell exhaustion in HIV infection.Applications Reported: This MBM7C7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MBM7C7 antibody has been pre-titrated and tested in intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|BATF, B-cell activating transcription factor, BATF1, basic leucine zipper transcriptional factor ATF-like, SF-HT-activated gene 2 protein, SFA-2, SFA2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.