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Basophil Marker, PE, clone: 2D7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9748-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15577226

  • POA / 100 tests

This item cannot be ordered online because the price is subject to change. Please contact our Product Support team on 01509 555500 for assistance.

Disclaimers:

For Research Use Only.


Description

Description

Description: The 2D7 monoclonal antibody recognizes human 2D7 antigen expressed exclusively by human basophils. This human basophil marker is a 7.2-7.5kDa protein present exclusively in the secretory granules of human basophils. Upon activation, basophils may degranulate, thereby decreasing the amount of 2D7 antigen available for staining. This antibody does not stain mast cells.Applications Reported: This 2D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 2D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This antibody can be used at 5μL (0.25μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Basophil Marker
2D7
PE
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
2D7
IgG1
RUO
Primary
Human
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