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Applied Biosystems™ TaqMan™ High-Capacity RNA-to-cDNA Kit

Designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR™ Green PCR Master Mix, and other PCR enzymes

Brand:  Applied Biosystems™ 4387406

Code : 50

Weitere Details : Weight : 0.01000kg

Artikelnummer. 10704217

  • £162.00 / Each

EU Stock 250

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For Research Use Only. All usage must comply with product instructions.
 

Beinhaltet: 50 Reaction Kit: 1 × 50μL of 20X Enzyme mix (MuLV and RNase inhibitor protein); 1 × 500μL of 2X RT Buffer Mix (includes dNTPs, random octamers, and oligo dT-16)

Beschreibung

Beschreibung

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit featuring:

  • Fast—short reaction time (typically 30–60 min.)
  • Convenient—easy workflow with few pipetting steps (2 tubes)
  • Reliable—reverse transcription of both abundant and limited targets
  • Flexible—optimized two-step protocol, enabling multiple PCR reactions from a single reverse transcription reaction
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

Streamlined cDNA synthesis while maintaining detection sensitivity The components work together to provide sensitive and specific reverse transcription across a broad range of template amounts. Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

PCR & Real-Time PCR, Reverse Transcription

Bestellinformation

Shipping Condition: Dry ice

Spezifikation

Spezifikation

Real-Time PCR
1 × 50μL of 20X Enzyme mix (MuLV and RNase inhibitor protein); 1 × 500μL of 2X RT Buffer Mix (includes dNTPs, random octamers, and oligo dT-16)
RNA
37deg.C
SuperMix or Master Mix
50 reactions
Store between -15deg. to -25deg.C
Videos
Dokumentation

For Research Use Only. Not for use in diagnostic procedures.