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Applied Biosystems™ SYBR™ Select Master Mix

Cost-effective solution for real-time PCR applications.

Brand:  Applied Biosystems™ 4472920

UNSPSC : 41106300

Code : VX

Additional Details : Weight : 0.00450kg

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Product Code. 13236529

  • £2085.00 / Pack of 10


EU Stock 41
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Description

  • Specific - minimize primer-dimer and non-specific amplification
  • Reproducible and sensitive - consistent amplification across a wide dynamic range
  • Bright - contains SYBR™ GreenER™ dye for maximum brightness
  • Carry-over contamination control - contains heat-labile UDG
  • Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity:
  • SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix
  • The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:
  • SYBR™ Select Master Mix is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA

Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
  • SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
  • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility:
  • SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system
  • It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems

Order Info

Shipping Conditions: Wet Ice

Description & Specifications

Specifications

Purity or Quality Grade: UP (Ultra Pure)
The 2X mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 x 1mL tube, sufficient for 100, 20μL reactions (10μL Master Mix per reaction).
Store at 2° to 8°C.
Detection Method: SYBR; PCR Method: qPCR
DNA (Genomic), cDNA
Real time PCR (qPCR), real time PCR-based gene expression profiling
10 x 5mL
Primer-Probe Compatability: Unlabeled Oligos
7500 Fast System, 7500 System, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Fast, Standard