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Applied Biosystems™ Fast SYBR™ Green Master Mix

Designed for speed, provides a fast, reliable and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range or PCR efficiency.

Brand:  Applied Biosystems™ 4385618

UNSPSC : 41106300

Code : 50

Additional Details : Weight : 0.00450kg

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Product Code. 10631376

  • £2610.00 / Pack of 10


EU Stock 79
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For Research Use Only. All usage must comply with product instructions.
 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Requires : Template and primers

Specifications

Description

  • Fast - Real-time PCR results in as fast as 35 minutes
  • Sensitive - Detect very low copies of target
  • Specific - Minimize primer-dimer and non-specific amplification
  • Reproducible - Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix
  • AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
  • SYBR™ Green I dye to enable detection of double-stranded DNA
  • Deoxynucleotides (dNTPs) to help maintain optimal PCR results
  • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
  • Passive internal reference based on proprietary ROX™ dye to enable increased precision
Description & Specifications

Specifications

Purity or Quality Grade: UP (Ultra Pure)
The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1 and optimized buffer components. 10 x 5mL vial sufficient for 5000 reactions of 20μL each.
Store at 2° to 8°C.
Detection Method: SYBR; PCR Method: qPCR
cDNA, DNA (Genomic)
Real time PCR (qPCR), real time PCR-based gene expression profiling
10 x 5mL
Primer-Probe Compatability: Unlabeled Oligos
7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Fast