missing translation for 'onlineSavingsMsg'
Learn More

Applied Biosystems™ Fast SYBR™ Green Master Mix

Designed for speed, provides a fast, reliable and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range or PCR efficiency.

Brand:  Applied Biosystems™ 4385616

Code : 50

Additional Details : Weight : 0.00450kg

 View more versions of this product

Product Code. 10556555

  • £560.00 / 1 set


EU Stock 379
Add to basket

For Research Use Only. All usage must comply with product instructions.
 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Requires : Template and primers

Description

Description

  • Fast - Real-time PCR results in as fast as 35 minutes
  • Sensitive - Detect very low copies of target
  • Specific - Minimize primer-dimer and non-specific amplification
  • Reproducible - Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix
  • AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
  • SYBR™ Green I dye to enable detection of double-stranded DNA
  • Deoxynucleotides (dNTPs) to help maintain optimal PCR results
  • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
  • Passive internal reference based on proprietary ROX™ dye to enable increased precision
Specifications

Specifications

Purity or Quality Grade: UP (Ultra Pure)
The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1 and optimized buffer components. 2 x 5mL vial sufficient for 1000 reactions of 20μL each.
Store at 2° to 8°C.
Detection Method: SYBR; PCR Method: qPCR
cDNA, DNA (Genomic)
Real time PCR (qPCR), real time PCR-based gene expression profiling
2 x 5mL
Primer-Probe Compatability: Unlabeled Oligos
7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Fast
Special Offers

Special Offers

  • This issue of Life in the Lab, “Illuminating the immune system”
    : 12863

    2017-12-31

    *Discount will apply to qualifying orders received by Fisher Scientific between 19/10/2017 and 31/01/2018. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

    Terms & Conditions