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Applied Biosystems™ Fast SYBR™ Green Master Mix

Designed for speed, provides a fast, reliable and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range or PCR efficiency.

Brand:  Applied Biosystems™ 4385618

Code : 50

Additional Details : CAS Number : 7732-18-5 Weight : 0.00450kg

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Product Code. 10631376

  • £2860.00 / Pack of 10

In stock, ready to ship: 30

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For Research Use Only. All usage must comply with product instructions.

Requires : Template and primers



  • Fast - Real-time PCR results in as fast as 35 minutes
  • Sensitive - Detect very low copies of target
  • Specific - Minimize primer-dimer and non-specific amplification
  • Reproducible - Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix
  • AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
  • SYBR™ Green I dye to enable detection of double-stranded DNA
  • Deoxynucleotides (dNTPs) to help maintain optimal PCR results
  • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
  • Passive internal reference based on proprietary ROX™ dye to enable increased precision


10 × 5 mL vials. The 2X mix contains SYBR® Green 1 Dye, AmpliTaq Gold® Fast DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1, and optimized buffer components. Sufficient for 5000 reactions of 20 µL each.

Store at -5 to -30°C.
ROX (Pre-mixed)
Unlabeled Oligos
UP (Ultra Pure)
7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
DNA (Genomic), cDNA
SYBR® Green
AmpliTaq® Fast DNA Polymerase, AmpliTaq® Fast UP
10 x 5mL

For Research Use Only. Not for use in diagnostic procedures.