High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

Brand: ABI Applied Biosystems

Manufacturer Part Number: 4374967


UNSPSC: 41106313

Code: 50

Additional Details:
Additional Details: Weight: 0.00450kg

Product Code. 10743647

Quantity Price
1 £ 1966.0 / 1 set
Estimated Shipment date
from Supplier 08-12-2016
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


For Use With (Application) cDNA Reverse Transciption, RT-PCR (Endpoint), Real-Time PCR
Format Separate Components
Includes 10X RT Buffer (2 × 1mL); 10X RT Random Primers (2 × 1mL); 25X dNTP Mix (100mM, 1mL); MultiScribe Reverse Transcriptase (1mL at 50U/μL); RNase Inhibitor (10 × 0.1mL at 20U/μL)
Quantity 1000 reactions
Sample Type RNA
Storage Requirements Store between -15deg. to -25deg.C
Temperature (Metric) Operating 37deg.C

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in a single 20μL reaction. This kit also includes two tubes of RNase inhibitor (100μL each). Reactions can be scaled up to 100μL to generate 10μg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

  • Linear target amplification for real-time PCR
  • Higher yields and precision than other cDNA synthesis kits—at a fraction of the cost
  • Ten-fold greater dynamic range than other kits
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

Extensively tested with a variety of templates Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

PCR & Real-Time PCR, Reverse Transcription