Invitrogen™ Rat IgG2a, Alexa Fluor 647, Isotype Control, Invitrogen™

Rat Isotype Control

Brand:  Invitrogen™ R2A21

Product Code. 10558383

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Specifications

• Antigen: Rat IgG2a
• Concentration: 100 ug/ml
• Formulation: PBS with 4mg/mL BSA and 0.1% sodium azide
• Host Species: Rat
• Purification Method: Purified
• Primary or Secondary: Isotype Control (Primary)
• Form: Purified

• Applications: Flow Cytometry
• Conjugate: Alexa Fluor 647
• Gene Alias: IgG2, IgG2a
• Immunogen: Rat IgG2a
• Quantity: 500 μL
• Content And Storage: Store at 4°C, in the dark
• Isotype: IgG2a