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Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 647, Polyclonal, Secondary Antibody, Invitrogen™

Donkey Polyclonal Secondary Antibody

Brand:  Invitrogen A31573

Code : 50

Additional Details : Weight : 0.00454kg

Product Code. 10543623

  • £256.00 / Each


EU Stock 22
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

To minimize cross-reactivity, these donkey anti-rabbit IgG whole antibodies have been affinity-purified and show a published cross-reactivity to rat IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye with excitation ideally suited to the 647 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10μg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
Specifications

Specifications

Rabbit IgG (H+L) Highly Cross Adsorbed
Polyclonal
Alexa Fluor 647
liquid
Gamma Immunoglobins Heavy and Light chains
Purified
RUO
Secondary Antibody
Polyclonal
Immunocytochemistry, Immunofluorescence, Immunohistochemistry
2mg/mL
PBS with 5mM sodium azide; pH 7.5
Donkey
IgG
500μL
4° C, store in dark
Secondary
Rabbit
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