Phospho-NCF4 (Thr154) Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA538379

Product Code. 15565301

Description

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Specifications

• Antigen: Phospho-NCF4 (Thr154)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NCF4
• Gene Alias: NCF, P40PHOX, SH3PXD4, NCF-4, SH3 and PX domain-containing protein 4, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, p40-phox
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17972, 4689
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 1 mg/mL
• Formulation: Dulbecco′s PBS with 150mM NaCl, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: Q15080, P97369
• Gene Symbols: NCF4
• Immunogen: A synthetic phosphopeptide derived from human p40 phox around the phosphorylation site of Thr154 (P-R-TP-R-K)
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG