Glucocorticoid Receptor Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA1512

Product Code. 11543581

Description

PA1-512 detects glucocorticoid receptor (GR) from human, mouse and rat tissues. PA1-512 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, ChIP and gel shift procedures. By Western blot, this antibody detects an ∽97 kDa protein representing GR as well as two other unidentified proteins at ∽135 to ∽145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate. Immunohistochemical staining of GR in rat hippocampus using PA1-512 results in staining primarily in the nucleus. PA1-512 does not disrupt receptor-DNA interactions. The PA1-512 immunogen is a synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR. The PA1-512 immunizing peptide (Cat. # PEP-002) is available for use in for neutralization and control experiments. Reconstitute in 50 μL PBS to create a stock of 1 mg/mL.

Glucocorticoid Receptor Polyclonal antibody specifically detects Glucocorticoid Receptor in Human, Mouse, Rat samples. It is validated for ChIP Assay, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot.

Specifications

• Antigen: Glucocorticoid Receptor
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Nr3c1
• Gene Alias: cytosolic/nuclear receptor; fb13f09; GCCR; Gcr; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Grl; Grl1; Grl-1; Nr3c1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858; utouto; utut; wu:fb13f09; zgc:113038
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14815, 24413, 2908
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: ChIP Assay, Gel Shift, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: antibody with no preservative
• Gene Accession No.: P04150, P06536, P06537
• Gene Symbols: Nr3c1
• Immunogen: Synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG