DYKDDDDK Epitope Tag Monoclonal Antibody, Clone: L5, Invitrogen™

DYKDDDDK Epitope Tag Antibody (L5), MA1-142, from Invitrogen™. Species Reactivity: Tag; Applications: ELISA, Western Blot.

Overview
Brand: Invitrogen

Manufacturer Part Number: MA1142

UNSPSC: 12352203

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15377364

Quantity Price
1 £ 160.0 / 100µg
Estimated Shipment date
from Supplier 04-04-2017
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Description and Specification

Specification

Applications Enzyme Linked Immunosorbent Assay
Applications Western Blot
Formulation 100μg purified antibody (1mg/mL) in PBS and no preservative
Antigen DYKDDDDK Epitope Tag
Storage Requirements Store at -20°C
Cross Reactivity Tag
Isotype IgG2a
Immunogen Recombinant fusion proteins of mouse Langerin/CD207
Primary or Secondary Primary
Format Purified
Clone L5
Conjugate Unlabeled
Host Species Rat
Species Reactivity Tag
Monoclonal or Polyclonal Monoclonal
Quantity 100μg
Gene Alias FLAG Tag, DYKDDDDK tag, FLAG antibody, ECS epitope tag, DDDDK epitope tag, DDDDK, DYKDDDDK epitope tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, ECS tag

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag's structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
Host Species: Rat
Cross Reactivity: Tag
Isotype: IgG2a
Clone: L5
Immunogen: Recombinant fusion proteins of mouse Langerin/CD207
Formulation: 100 ug purified antibody (1mg/mL) in PBS and no preservative
Synonyms: FLAG Tag, DYKDDDDK tag, FLAG antibody, ECS epitope tag, DDDDK epitope tag, DDDDK, DYKDDDDK epitope tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, ECS tag
The MA1-142 antibody (clone L5) has been successfully used as a detection antibody in a direct ELISA with FLAG-tagged protein-coated plates. Similar Epitope as the FLAG tag from Sigma. The MA1-142 immunogen is recombinant fusion proteins of mouse Langerin/CD207, which contained a flexible linker sequence from E. coli OmpF and a FLAG epitope. (J Immunol Methods. 2008 Feb 29;331(1-2):27-38. Epub 2007 Nov 26.)