CD28, Functional Grade, clone: CD28.2, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-0289-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis. CD28.2 has also been reported in costimulation of T cells in in vitro functional assays.Applications Tested: TheCD28.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|aqueous buffer, no sodium azide|
|Store at 2-8°C.|