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6x-His Tag Mouse anti-Tag, Clone: HIS.H8, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA121315

Code : LE

Additional Details : CAS Number : 69-74-9 Weight : 1.24740kg

Product Code. 11533923

  • £159.00 / 100µg

EU Stock 65

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Description

Description

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombit proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombit protein using genetic engineering. By choosing a particular epitope and recombit protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombit proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Specifications

Specifications

6x-His Tag
Monoclonal
1 mg/mL
PBS with no preservative; pH 7.2
His Tag; Histidine Tag; Histidine Epitope Tag; 6X His eptope tag; 6His tag; 6X His
6x His synthetic peptide.
Protein A
RUO
Antibody
Monoclonal
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
HIS.H8
Unconjugated
Liquid
Mouse
IgG2b
100 μg
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Primary
Tag
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